5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
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At that time, the early indications were being dismissed as particles, however the retrospective evaluation shown shared features While using the problematic cells in society. Tracing the an infection served the lab establish additional laboratory treatments for screening and security of assay-critical mobile lines.
As samples are obtained to the Attune CytPix Flow Cytometer, the large-pace brightfield digital camera captures and shops illustrations or photos of detected occasions in a amount as much as six,000 visuals per next, with regards to the flow level and picture sizing. For greater adaptability, Attune Cytometric Application allows you to modify the impression capture frequency as desired and select a particular gate for impression capture, if demanded.
(B) Uncooked 264.7 macrophages are often annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The volume of singlets available for Investigation raises once the person trains and employs a more correct model.
Learn new insights into your experimental data by visualizing populations making use of merged fluorescence and morphology info. Our application interface wizards enable downsampling, concatenation (merged documents) and dimensionality equipment which might be created into the application for an intuitive, streamlined info Assessment working experience.
"A picture isn't pretty worthy of one thousand dots, but it surely definitely really helps to see photos. It is great to see photos that correlate with staining. By way of example, AnnexinV-positive cells which are morphologically distinctive than healthful cells, or CD14+ monocytes that happen to be much larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it truly is illuminating to see what number of doublets slip by way of traditional singlet gates, and excellent to confirm the position of surprising double-positive activities in my Assessment."
Pair your Attune flow cytometer by having an autosampler for enhanced workflow effectiveness, and incorporate robotic integration for optimum throughput.
Constant image excellent even at higher throughput Acoustic focusing along with a substantial-pace camera Blend to image these T cells consistently at lower or higher flow costs. Simply modify emphasis and camera options to fulfill experimental demands.
The Attune NxT cytometer could be the dependable workhorse instrument your lab can rely on for dependable and dependable effectiveness. The Attune CytPix model includes a high-speed brightfield digital camera and AI-pushed image Assessment software, with recent updates such as device Studying equipment and an intuitive user interface, enabling Improved facts precision, accuracy, and more rapidly insights into sample biology.
Probably most of all, these situations contain cells of Evidently distinctive phenotypes which get more info may lead to incorrect conclusions with regards to double favourable activities (particularly in rare populations).
Seem waves allow precise cell alignment, leading to Extraordinary details high quality and ~10X more quickly sample flow costs.
Software Observe: Automated graphic Evaluation reduces person-to-user variability in flow cytometry gating approaches
Leverage the power of Highly developed engineering and innovation to accelerate your discoveries. Have confidence in while in the Attune Flow Cytometer family members to deliver the trustworthiness, pace, and accuracy that established your function apart.
No wash/no lyse leukocyte Evaluation with imaging. Cells have been obtained from 24-hour-previous blood by dilution in 1 mM EDTA ( Skilled UX/UI enables customers to train AI styles for expanded apps. (A) The “Coach Product” attribute delivers annotation applications and true-time product education precision.
Gating on these prolonged parameters lets you immediately and accurately establish populations of interest and confirm gating tactic with little or no guide evaluation. The Attune application delivers entry to condition-of-the-art machine Understanding analysis applications in an expertly designed consumer interface, making it extremely user friendly.
Because the mobile line had Formerly been analyzed to the Attune CytPix Flow Cytometer, the scientists went back again to the images and were being in the position to doc the microbial infection at least five days before.
The Attune CytPix Flow Cytometer permits users to visualize samples throughout acquisition, validate the accuracy of gating procedures, and assist results with responsible documentation for publication applications.
What appeared to be a homogeneous inhabitants is definitely extra diverse—an insight that needs to be regarded when interpreting final results.